H5N1亚型禽流感病毒感染番鸭引起肺损伤和脑损伤的机制探索

doi:10.11843/j.issn.0366-6964.2016.05.013

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (5): 962-969.doi: 10.11843/j.issn.0366-6964.2016.05.013

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The Mechanism of Lung Injury and Brain Injury in Muscovy Ducks Infected with H5N1 Avian Influenza Virus

LI Qi^1，2，3# ，YE Yu^5# ，TONG Tie-zhu^6# ，LIU Jie^1，2，3，LIU Yang^1，2，4，ZHANG Zhen^1，2，4，MIAO Pei-si^1，2，4，GAO Ying-qi^1，2，4，ZHANG Jiao^1，2，4，FAN Hui-ying^{1，2，3，4*} ，LIAO Ming^{1，2，3，4*}

(1.College of Veterinary Medicine，South China Agricultural University，Guangzhou 510642，China；
2.National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control，Guangzhou 510642，China；3.Key Laboratory of Animal Vaccine Development，Ministry of Agriculture，Guangzhou 510642，China；4.Key Laboratory of Zoonosis Prevention and Control of Guangdong Province，Guangzhou 510642，China；5.College of Animal Science and Technology，Jiangxi Agricultural University，Nanchang 330045，China；6.Huizhou Entry-Exit Inspection and Quarantine Bureau，Huizhou 516006，China)

Received:2015-10-22 Online:2016-05-23 Published:2016-05-23

Abstract

Abstract:

This experiment was conducted to preliminarily investigate the mechanisms of lung injury and brain injury in duck infected with avian influenza virus.Ducks were intranasally infected with DK383 virus (A/Duck/Guangdong/383/2008) and DK212 virus (A/Duck/Guangdong/212/2004)，respectively.The lung and brain were collected at 1，2，and 3 days post infection (dpi) for pathological observation，RNA extraction and detection of the proliferation of virus.The expression of several genes mRNA were detected by RT-PCR.DK383 was highly pathogenic with high mortality rate of 70% and severe neurological sign.The histopathological observation of duck infected with DK383 showed extensive neuronal degeneration and necrosis occurred in the brain tissue，severe acute congestion and hemorrhage in the lung.Compared to DK212，DK383 replicated more efficiently in the lung and brain and activated TLR-TRIF-TRAF6 signaling more strikingly.The improper regulation of DK383-induced immune response and the viral replication competent are correlated with the severity of the disease.

CLC Number:

S858.325.3

LI Qi，YE Yu，TONG Tie-zhu，LIU Jie，LIU Yang，ZHANG Zhen，MIAO Pei-si，GAO Ying-qi，ZHANG Jiao，FAN Hui-ying，LIAO Ming. The Mechanism of Lung Injury and Brain Injury in Muscovy Ducks Infected with H5N1 Avian Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 962-969.

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The Mechanism of Lung Injury and Brain Injury in Muscovy Ducks Infected with H5N1 Avian Influenza Virus

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